中国株单纯疱疹病毒胸苷激酶基因及其应用
本发明通过ＰＣＲ方法克隆出高活性的中国株单纯疱疹病毒１型胸苷激酶（ＴＫc）基因，并测定了其ＤＮＡ序列，构建了含该基因的逆转录病毒重组表达载体pLTKcSN，并完成了ＰＡ３１７（PLTKcSN)包装细胞株的建立。还应用该包装细胞及其产生的“假型”病毒与ＧＣＶ配合来治疗恶性肿瘤，在体外的细胞实验及体内的动物试验中，表现其对肿瘤细胞具有杀灭和抑制作用。
发明专利
CN96116231.7
1996-01-25
CN1134982
1996-11-06
C12N15/54
上海市肿瘤研究所
顾健人; 郭忠; 许秀兰
200032上海市斜土路2200弄25号
上海华东专利事务所
衷诚宣
上海;31
一种单纯疱疹病毒1型胸苷激酶基因，其特征在于它是具有中国株单纯疱疹病毒1型胸苷激酶（TKc）基因， 其CNA的核苷酸序列及其所编码的氨基酸序列如下：a： TKc 基因的氨基酸序列b： TKc 基因的核苷酸序列a： 1 Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Alab： 1 ATc GCT TGC TAc ccc TGC CAC CAA CAC GCG TCT CCC TTc GAC CAC GCT17 Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg49 GCC CGT TCT CGC GGC CAT AGC AAC CGA CGT ACC GCG TTG CGC CCT CGC33 Arg Gln Gln G1u Ala Thr G1u Val Arg Pro Glu G1n Lys Met Pro Thr97 CGG CAG CAA GAA GCC ACG GAA GTC CGC CCG GAG CAG AAA ATA、ATG CCC ACG49 Leu Leu Arg Val Tyr Ile Asp G1y Pro His G1y Met Gly Lys Thr Thr145 CTA CTG CGG GTT TAT ATA GAC GGT CCC CAC GGG ATG GGG AAA，ACC ACC65 Thr Thr Gln Leu Leu VaI Ala Leu Gly Ser Arg Asp Asp lle Val Tyr193 ACC AGG CAA CTG CTG GTG GCC CTG GGT TCG CGC GAC GAT ATC GTC TAC81 VaI Pra Glu Pro Met Thr Tyr Trp Arg Val Leu G1y Ala Ser Glu Thr241 GTA CCC GAG CCG ATG ACT TAC TGG CGG GTG CTG GGG GCT TCC GAG AcA97 11e Ala Asn lle TYr Thr Thr Gln His Arg Leu Asp Gln G1Y G1u I1e289 ATC GCG AAC ATC TAC ACC ACA CAA CAC CGC CTC GAC CAG GGT GAG ATA113 Ser Ala G1y Asp Ala Ala Val Val Met Thr Ser A1a G1n I1e Thr Met337 TCG GCC GGG GAG GCG GCG GTG GTA ATG ACA AGC GCC CAG ATA ACA ATG129 Gly Met Pra TYr Ala VaI Thr Asp Ala VaI Leu Ala Pro His l1e G1y385 GGC ATG CCT TAT GCC GTG ACC GAC GCC GTT CTG GCT CCT CAT ATC GGG145 Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu I1e433 GGG GAG GCT GGG AGC TCA CAT GCC CCG CCC CCG GCC CTC ACC CTC ACC CTC ATC16 Phe Asp Arg His Pro lle Ala Ala Leu Leu CYs TYr Pro Ala Ala Arg481 TTC GAC CGC CAT CCC ATC GCC GCC CTC CTG TGC TAC CCG GCC GCG CGA177 Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala529 TAC CTT ATG GGC AGC ATG ACC CCC CAG GCC GTG CTG GCG TTC GTG GCC193 Leu lle Pro Pro Thr Leu Pra Gly Thr Asn lle VaI Leu G1y Pro Leu577 CTC ATC CCG CCG ACC TTG CCC GGC ACC AAC ATC GTG CTT GGG CCC CTT209 Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly Gly625 CCG GAG GAC AGA CAC ATC GAC GAC CGC CTG GCC AAA CGC CAG GGG CCC GGC225 Glu Arg Leu Asp Leu Ala Met Leu Ala Ile Arg Arg Val Trg Val Tyr Gly673 GAG CCG CTG GAC CTG GCT ATG CGG CTG GCT GCG ATT CGC CGC GTT TAC GGG241 Leu Leu Ala Ash Thr Val Arg Tyr Leu Gln Cye Gln Gly Gly Ser Trp Arg721 CTA CTT GCC AAT ACG GTG CGC TAT CTG CAG TGC GGC TCG TGG CGG GGG257 Glu Asp Trp Arg Gln Lau Ser Gly Thr Ala Val Pro Pro Gln Gln Gly Ala769 GAG GAG TGG AGA CAG CTT TCC GGG ACG GCC CTG CCG CTG CCC CAG GGT GCC273 Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu817 GAG CCC CAG AGC AAC GCG GGC CCA CGA CCC CAT ATC GGG GAC ACG TTA269 Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Aep Leu865 TTT ACC CTG TTT CCG GCC CCC GAG TTG CTG GCC CCC AAC GGC GAC CTG305 Tyr Asn Val Phe Ala Trp Ala Leu Ast Val Leu Ala Lys Arg Leu Arg913 TAT AAC GTG TTT GCC TGG GCC TTG GAC GTC TTG TCC AAA CGC CTC CGT321 Ser Met His Val Phe Ila Leu Asp Tyr Asp Gln Ser Pro Ala Gly Dys961 TCC ATG CAC GTC TTT ATC CTG GAT TAC GAC CAA TCC CCC GCC GGC TGC337 Arg Asp Ala Leu Leu Gln Leu Thr Sar Gly Met Val Gln Thr His Val1009 CGG GAC GCC CTG CTG CAA CTT ACC TCC GGG ATG GTC CAG ACC CAC GTC350 Thr Thr Pro Gly Ser Ila Pro Thr Ila Cys Asp Leu Ala Arg Thr Phe1057 ACC ACC CCA GGC TCC ATA CCG ACG ATC AGC GAC CTG GCG CGC ACG TTT369 Ala Arg Glu Met Gly Glu Ala Asn ***1105 GCC CGG GAG ATG GGG GAG GCT AAC TGA。