可促进肿瘤细胞分化的cDNA序列及其真核表达载体及应用
本发明涉及一种具有促进肿瘤细胞分化功能的cDNA序列、含有该序列的真核表达载体以及该序列在制备基因治疗肿瘤的制剂中的应用。所述的cDNA序列是从由全反式维甲酸处理的人食管癌细胞系ＥＣ８７１２中分离得到。
发明专利
CN95101293.2
1995-02-07
CN1128798
1996-08-14
C12N15/11
中国医学科学院肿瘤医院% 中国医学科学院肿瘤研究所
吴∴; 冯骆; 王志华; 王秀琴
100021北京市朝阳区潘家园
永新专利商标代理有限公司
程伟
北京;11
一种具有促进肿瘤细胞分化功能的cDNA序列，其特征是所述的cDNA序列为：GGATCCTCTC GGGGAAACTG AGTTCCAGTG TCATGGGTGA ACAACAGAAG AGACTAGCTA 60ATTCTTGTAT ATCCCAGACC AAGCTGAAAG CTCCTCCCAC TTCACCCCTC ATCGTGATTG 120TTATCTATAA ATTTGATTGC ACAATGTTGC AGACCCCTGG GACTCCAGAT TGGCTGGGAG 180AGCCTTCCTG TGTTATGGTC AAGATCAGCC TGACTTTCTG GAAGGAAGCT GGCCGATGTC 240CTGTTTTCAG ACCATTCCCT GGCTTGGTAA AGCTGAGAAG TGCCCAGATA GGGAGCTGTG 300ATACTTCGAT TCCATCCCCA CAGCCAAATC TGAATCTCTA AACTTGTTTC CTGATCATTT 360GGAACAGGAG TTGTTTTCCT AATGAGGAAT GCTTTCCTCT ATTCTTTGGG CTTGCCTGGA 420CTGAGTGATT CAACCTAACA CCTTCCCAGG TCTCAAGTGA GTCACCCCAG GGCTGCACTC 480CAGCCTAGGC TCACAAAGGG GAAAAAGAGG GAACTATTTA AGAGCACCCC CTGCTCCCCC 540CTTTTGCTAA TCCTTTGAAC ATCTCAAAGT ATTTCAAACT ACTACTGGGG TTTGTGTTTT 600CTTTTGAATG TAGGATAGAG GGTGTAACAG TTTGAAGCTG AGGGTGAGTC TAAAAGAGCC 660AAAAATAAGA TCAAAAACTA AATGTTAAAC GCTTCAATAG ATCATGGGGG GGGGGTGTCA 720AAGATAGTAA TTACTTATTG GTTTATGAAT GATGGTGAAT TAAAGTCCCA AAGACACTCC 780AGGATGCTAG AGACCTTCTC TGAGCAAATG GGGTCATTTA GGACATTTAG GGTACCTTTC 840TCCCTGGACT TTACCTACAT GTTTCAGTTT CTGGTTTGGA TTGTGGCTTT TCAGAHHCTT 900ATAATTAATA AGATACCTGT TCCAAAAGGC TTTCTCATGG GCCATTGATG AGCGTGTGTC 960ATGAGTGTGA GAAAGTGCTT GGGAGGAAGG CGAGCTTGCA ATTTCTAAAA TAATGACTCA 1020TGTAAAACAA GGTTTTATTT TATGATCTGA CCAGTAGCAC TTCTAAACTG GCTTCTTCCC 1080AGGAGCAAGC AGGGATCTGA TTTTAAAAAC TAAGTGGGTA AGGTAGGAGT GAGCTATGTG 1140CCTCTGAGTA CACACAACAC CCTCCACCCC AACCCCCCAA TCCACAAACA AGGAGGGGAA 1200CTGAAGTCTA CCAGTTCTTG AGTAGTGAAG CTTACAGTCC CAAAGCCCCA GCCAAGGTTG 1260TGAGGTTAGG CTTTGAGCAT GCATTTTAAT TCCAGCGCAT CAGTTCTGTC AGAAGGAACC 1320GTTCTCCTTA CTCTCACGAG AGATTCCAGC TCCTCCTCGA GTTAGGTCAG TTTATGCACC 1380AGAGTTTCGA AGCTGTTCGA GTTTGTGTTT CAACTGTTCT CGTCGTTTCC TCAATAAGTC 1440CTTTTCAGAG GTGAGCTTGT GCTCGTCTGC TTGAATGGAC AGGATGTAGG CGGTGGCTTT 1500TTTGAGGATC ACTACCTTGG GGGCCTTTTC GTTGTTTTCC AATTCAGGGA TCTGGTCACG 1560CAGGGCAAAA AAGCTGCGCT TCAGCTCGTT CCTCCTCTGA CGTTCCAAGA CGTTGTGTGT 1620CTGATCTGCT TCAGGACCCT GCCACTGTCC AACTTGGCCC TCTTGGCAGC TGGATAGTCC 1740TTCCTTGTGG AGGGGGGTGC GGCGTAGTTG TGCTGGTGAG TGGAGACGTG GCACCTCTTG 1800AGGACCAGTG GGCTGTGCGG AGGTTTGCTG TGGCCTCGGG ATGGAGATGA GCCCGACTCC 1860GACCTCTTGG CAGGGGTTTG CCTCTTCTCC ACAGACACCA CATCAATTTC TTCCTCATCT 1920TCTTGCTCTT CTTCTGTAAG GGAGGAAGTG GAAGATCACA GTTAGCCACG CCTCCTGAAT 1980CCCGGACGCT ATACATAGGA TTTGACCGAT GCTATCCAAA TACCAAGTTG GAAAGGGAAG 2040AAAGTAAGAA TCAGATCCAG ACACCATCTT CCCTTCTCCC AGAAAGGCAG ACAGGGTTAG 2100GGCACAGGTG AGAAATTACC ATATGGTATC ATATGGTATC CTTGCCAATT CTTACCTAAG 2160ATTTAATGAG GCTTCAGACA CACCCAAAGC AAAGCACATT TCCCAAGCAC CTCCTATTTT 2220TAAGGGACTT TACCGAGAAC CTTTCAGGTC CCGTGAGGGA TAGCTGCAAA TCTCTTGCTA 2280TGACTTTAGC AACTCCCTAT TTTACTGGAA GCACAATTAA AAAAAAAAAA AAAAAAAAAA 2340GTCTTGCAGC TTTGCCAAAC GTCCTGGTGT TGGGGGAGTG TTGGGACGGG GGGAGAAGTC 2400CAAATTGAAC AGAATTTGCT TTGAAGTGTT CAAAAGAGGA CGAAAAAACA AAGCAAAACA 2460AAACACCCAA AAGAAGCCAG CTCTTCAATT CTTTAGGAAC TTTCACTAGT GCCTGGCTCT 2520AAGGCTATAA AGGCTATCCC AGCTTCTGTC ATTGCTTCTC TTGGGTCTTA GACAAACGTA 2580TCCATTAAAT TCAAATATTC TTCTGGCAGT CAAGAAAAGA AGCAGGTAAT TCACCCCCCT 2640CCAGATCAGT TCCTTTATCC TCATTCACAC CCTTCCCAGG GTAATTAAAA TGCTTAGGAG 2700GGGTAAAGGG AGTGGATGGA GTCGAAAGAT AAGCCAGAAG ATTTAAAAAC CCAAATGAAA 2760TTCCTGAGGG GTGGGAAGGG TGGGGGGTGG GGGTGACAAC CAGGGCGGTC TGGGGGGGGG 2820GGGGAGGGGA GGGAGGGATA GCTGAAGCAC GGGAATCCAG GGATGAAGAG CTCTTAGACA 2880GAAGGGAAAG AAAGGGGGAG CCCCGGAGGC CAATTGAATT AATGCCAATG AGTGGTGGAG 2940AAGGAGGTCC ATCCAACCTC TCGCTTCCTA CCCTGCTGTG AATGGGGTAG CTTACCAGAG 3000TCGCTGCTGG TGGTGGGCGG TGTCTCCTCA TGCAGCACTA GGGGCTCAGG GCTGGCCCGT 3060GGGGAGGACT CGGAGGACAG CAGCGAGTCC GAGGAAGGAG AGAAGGCCGT GGAATCGGAC 3120GAGGTACAGG ATTTGGGCGA GCTGCTGTCG TTGAGCGGGT AGGGAAAGAC CACTGAGGGG 3180TCAATGCACT CGGACGCGGC GGCGGTGAGG TCCTGCAGGT ACAGGCTGGA GGTGGAGCAG 3240ACGCTGTGCC CGCGGGCGGG GCTCAGGCTG GTGCTGTCTT TGCGCGCAGC CTGGTAGGAG 3300GCCAGCTTCT CCGAGACCAG CTTGGCAGCG GCTGAGAAAC CGCTCCACAT ACAGTCCTGG 3360ATGATGATGT TCTTGATGAA GGTCTCGTCG TCAGGATCGC AGATGAAGCT CTTGTTCACC 3420ATGTCTCCTC CAAGTAACTC GGTCATCATC TCCAGCTGAT GATCGGCGGT GGAGAAGTTG 3480CCACCGCCGC CGTCATCGTC TTCCCTTGGG GAGAAGGACG TAGCGACCGC AACATAGGAT 3540GGAGAGCAGA GCCCGGAGCG GCGGCTCGGG GACAGGGGCG GGGTGGGAAG CAGCTCGAAT 3600TAGAAATTCT CTTCCTCGTC GCAGATGAAA TAGGGCTGTA CGGAGTCGTA GTCGAGGTCA 3720TAGTTCCTGT TGGTGAAGTT CACGTTGAGG GGCATCGTCG TGGCTGTCTG CTGGAGGGGG 3780CACAGCAAGG GAAGCAAGTC TAGAG 3805。