表达人蛋白C糖基化突变体的载体和化合物

摘要：

本发明描述了一种重组产生高活性酶原型人蛋白Ｃ的方法。这些酶原形式不同于天然蛋白Ｃ，它们有更高的酰胺解和功能活性及新的糖类结构。还公开了用于该方法的ＤNＡ化合物、载体和转化株。

专利类型：发明专利

申请/专利号：CN91101201.X

申请日期：1991-02-23

公开/公告号：CN1055560

公开/公告日：1991-10-23

主分类号：C12N15/09

申请/专利权人:伊莱利利公司

发明/设计人:布鲁斯·E·格里茨; 布赖恩·威廉·格林内尔

主申请人地址:美国印第安纳州

专利代理机构:中国专利代理有限公司

代理人:汪洋

国别省市代码:美国;US

权利要求：

一种制备重组DNA载体的方法，该方法包括将（I）含有一种蛋白编码顺序的重组DNA化和物和（II）含有一种启动子的DNA化合物连接起来，以便启动子在连接后定位驱动所述编码顺序的表达，所述蛋白质从氨基端到羧基端的氨基酸残基顺序如下：MET TRP GLN LEU THR SER LEU LEU LEU PHE VAL ALA THR TRP GLY ILESER GLY THR PRO ALA PRO LEU ASP SER VAL PHE SER SER SER GLU ARGALA HIS GLN VAL LEU ARG ILE ARG LYS ARG ALA ASN SER PHE LEU GLUGLU LEU ARG HIS SER SER LEU GLU ARG GLU CYS ILE GLU GLU ILE CYSASP PHE GLU ALA LYS GLU ILE PHE GAN ASN VAI ASP ASP THR LEUALA PHE TRP SER LYS HIS VAL ASP GLY ASP GLN CYS LEU VAL LEU PROLEU GLU HIS PRO CYS ALA SER LEU CYS CYS GLY HIS GLY THR CYS ILEASP GLY ILE GLY SER PHE SER CYS ASP CYS ARG SER GLY TRP GLU GLYARG PHE CYS GLN ARG GLU VAL SER PHE LEU R1 CYS SER LEU ASP ASNGLY GLY CYS THR HIS TYR CYS LEU GLU GLU VAL GLY TRP ARG ARG CYSSER CYS ALA PRO GLY TYR LYS LEU GLY ASP ASP LEU GLN CYS HISPRO ALA VAL LYS PHE PRO CYS GLY ARC PRO TRP LYS ARG MET GLU LYSLYS ARG SER HIS LEU LYS ARG ASP THR GLU ASP GAN GLU ASP GLN VALASP QRO ARG LEU ILE ASP GLY LYS MET THR ARG ARG GLY ASP SER PROTRP GLN VAL VAL LEU LEU ASP SER LYS LYS LYS LEU ALA CYS GLY ALAVAL LEU ILE HIS PRO SER ThP VAL IEU THR ALA ALA HIS CYS MET ASPGLU SER LYS LYS LEU LEU VAL ARG LEU GLY GLU TYR ASP LEU ARG ARGTRP GLU LYS TRP GLU LEU ASP LEU ASP ILE LYS GLU VAL PHE VAL HISPRO R2 TYR SER LYS SER THR THR ASP ASN ASP ILE ALA LEU LEU HISLEU ALA GLN PRO ALA THR LEU SER GLN THR ILE VAL PRO ILE CYS LEUPRO ASP SER GLY LEU ALA GLU ARG GLU LRU ASN GLN ALA GLY GLN GLUTHR LEU VAL THR GLY TRP GLY TYR HIS SER SER ARG GLU LYS GLU ALALYS ARG R3 ARG THR PHE VAL LEU ASN PHE ILE LYS ILE PRO VAL VALPRO HIS R4 GLU CYS SER GLU VAL MET SER ASN MET VAL SER GLU ASNMET LEU CYS ALA GLY ILE LEU GLY ASP ARG GLN ASP ALA CYS GLU GLYASP SER GLY GLY PRO MET VAL ALA SER PHE HIS GLY THR TRP PHE LEUVAL GLY LEU VAL SER TRP GLY GLU GLY CYS GLY LEU LEU HIS ASN TYRGLY VAL TYR THR LYS VAL SER ARG TYR LEU ASP TRP ILE HIS GLY HISILE ARG ALP LYS GLU ALA PRO GLN LYS SER TRP ALA PRO－COOH。